ISO 13307 INTERNATIONAL STANDARD First edition 2013-03-01 Microbiology of food and animal feed - Primary production stage Sampling techniques Microbiologiedes aliments-Stade de production primaire- Techniques deprelevement ERIE SO IsoRefegerrceourbey IHS under NotforR ed without license from IHS @ISO2013 IS013307:2013(E) COPYRIGHTPROTECTEDDOCUMENT IS02013 AHbyghty raeansedlalestronicotherwipemifieldlanilaitdfitdisgpplbbicatbpyimgyoltqnsthgintprndtioedntnttiaiaet) otimeamyisowithout prior written permission. Permission can be requested from either ISO at the address below or Iso's member body in the country of the requester. SSeostlight officeCH-1211 Geneva 20 Tel.+ 4122749 0111 Fax + 41 22 749 09 47 Wmaw [email protected] Published in Switzerland IHS undet University of Alt 4001, User=sharabiani, shahramfs No reproduction or networking permitted without license from IHS IS013307:2013(E) Contents Page Foreword iv Scope 1 2 Normativereferences 1 3 Terms and definitions 1 4 General arrangement 1 4.1 General 1 4.2 Sampling personnel 1 5 Diluents and disinfectants. 2 6 Apparatus and materials 4 7 SamplingtechniquesGeneralrecommendations 5 8 Samplingtechniques inthefarm environment 5 8.1 Samples taken after cleaning and disinfection 5 8.2 Surface sampling 6 Rudyes sgg Sampling floors. 6 8.5 Water samples 8 8.6 Moore'sdrainswabsortamponswab samples 9 9 Techniques fortaking samples from animals 9 9.1 Sampling from animals on the farm .9 9.2 Sampling animals at slaughter 10 10 Sampling techniques in the hatchery 12 10.1 General .12 10.2 Hatcher basket liner samples 12 10.3 Babdereggfshells 12 10.5 Meconium 13 10.6 Swabs from hatcher baskets 13 18:9 Dastnteshellamhreryswaste 13 10.9 Cull chicks .14 10.10 Samples from chick deliveries on the farm 14 11 Techniques for sampling animal transport vehicles, modules,and crates 14 11.1 General 14 Vehiclesfor large animals (cattle, sheep,pigs,horses) .14 11.2 Transporttransport modulesaftercleaning. ..14 11.3 12 Storageand transport of samples. .15 12.1 General recommendations 15 12.2 Recommendationsforsensitive organisms 15 Copyright Intemational Organization for Standardizatior Noefosre iii IS013307:2013(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards thudiegh(IsO tectmicet boabiasjtahsewofkf ptepbreingbodyInitteresitedailhStandebjdsti sonowhidhycarrtiechaital committeehasbeen establishedhastherighttobe represented onthat committee.International organizations, governmental and non-governmental, in liaison with Iso, also take part in the work. IsO collaborates closely with the electrotechnical standardization. International Electrotechnical Commission (IEC) on all matters of International Standards aredrafted in accordance with therules given in the ISO/IEC Directives, Part 2. 3tanmainstaak pfedbechnical techniabescommitteesprepare Intersidetedal Starthar ae mbarBrafernatfon xpting. Publication as an International Standard requires approval by at least 75 % of the member bodies castingavote. Aatentionrights.tfawrfshthf hetpeksibilftyaespmfsibltharelaerAitsfg anyltiskchidatreht mayrights.the subject of ISO 13307was prepared by the European Committee for Standardization (CEN) in collaboration with Agr erenicahtechnifaleTCopeFatfoRrBeustelSund GENtteeVienharMemeBtlogy, in accordance with the ational Organization for Standardizatio Uisub2U hghra"All rights reserved IS0 13307:2013(E) INTERNATIONALSTANDARD Microbiology of food and animal feed - Primary production stage Sampling techniques 1 Scope This International Standard specifies sampling techniques within the primary food-animal production stage, for detection or enumeration of viable microorganisms with particular reference to food-borne pathogens. This International Standard is not intended for use in diagnosis of animal disease. 2Normativereferences The followingreferenced documents are indispensable for the application of this document.For dated defeurenes@nlylthtiaggditiorca