ISO INTERNATIONAL STANDARD 11268-3 Second edition 2014-10-15 Soil quality Effects of pollutants on earthworms- Part 3: Guidance on the determination of effects in field situations Qualitedusol-Effetsdespolluantsvis-a-visdesversdeterre Partie3: Lignes directrices relatives a la determination des effets sur site Reference number ISO 11268-3:2014(E) @ IS0 2014 Intermational Crganization for Standardization Zhejian ng Institute of Standardization 5956617 rovided by IHS under lic se with ISO Not for Resale, 2015/5/13 08:09:37 IS0 11268-3:2014(E) COPYRIGHTPROTECTEDDOCUMENT @IS02014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,or posting on the internet or an intranet,without prior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. ISO copyright office Case postale 56·CH-1211Geneva20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland Drganization for Standardization Heene-zhejang lnenute a Standardzation @o1S9 2014 - All rights reserved Provided by IHS under e with IS Not for Resale, 2015/5/13 08:09:37 networking permited without icanse from IHs IS011268-3:2014(E) Contents Page Foreword ..iv Introduction .. 1 Scope. 2 Normative references ... 3 Units. 4 Principle. ..1. 5 Reagents and material .2 6 Apparatus .2 7 Procedure .2 7.1 Samplingofearthwormpopulations .2 7.2 Preservation .3 7.3 Determinationofbiomass .3 8 Preparationforthetest .3 8.1 Test site .3 9 Procedure .6 9.1 Application of test substance. 16 9.2 Sampling dates. .6 9.3 Reference substance. 7 Data assessment. 7 10 10.1 Endpoints. .7 10.2 Identification ofearthworm species :8 10.3 Determination ofbiomass withgutcontent. .8 11 Calculationandexpressionofresults 12 Validity of the test ..8 13 Test report. ..9 AnnexA(informative)Additional requirementsofpesticidetesting ..10 Annex B (informative) Information on specific earthworm species or communities in ..11 differentclimaticorgeographicregions ..12 Bibliography ili LicenseeZhejang Institute of Standardization 5956617 Not for Resale, 2015/5/13 08:09:37 without license from IHS IS0 11268-3:2014(E) Foreword Iso (theInternational Organization for Standardization)is a worldwidefederation of national standards bodies (so member bodies).The work of preparing International Standards is normally carried out through Iso technical committees.Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations,governmental and non-governmental, in liaison with IsO, also takepart in the work. ISo collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnicalstandardization. The procedures used to develop this document and those intended for its further maintenance are described in the Iso/IEc Directives, Part 1.In particular the different approval criteria needed for the different types of Iso documents should be noted. This document was drafted in accordance with the editorialrules of theIso/lEC Directives, Part2 (see www.iso.org/directives). Attention isdrawntothepossibilitythat some ofthe elements of this document maybethesubject of patentrights.IsO shall notbe held responsible for identifying any or all suchpatent rights.Details ofany patent rights identified during the development ofthe document will be in the Introduction and/or on the Iso list of patent declarations received (see www.iso.org/patents). Any trade name used in this document is information given for the convenience of users and does not constituteanendorsement. For an explanation on the meaning of Iso specific terms and expressions related to conformity assessment,as wellasinformationaboutISO'sadherencetotheWTOprinciples